| Base: |
Plasmid collection |
| Number in base: |
631 |
| Plasmid name: |
pMLO42 |
| Species: |
Escherichia coli K12 |
| Strain: |
DJ125 (=C600 recA) |
| Genotype: |
supE44 hsdR17 thr-1 leuB6 lacY1 thi-1 tonA21 recA
|
| Plasmid Sequence: |
on request |
| Construction: |
Insertion of ~ 3.7 kb long EcoRI-EcoRI fragment of GG124 (=HP45 ΩcmR) in place of EcoRI-EcoRI fragment of pMLO7 containing the spec/str resistance cassette.
|
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
cm |
| Antibiotic concetration: |
|
| Plasmid size: |
~ 6.1 kb |
| Origin of replication/Incompatibility group: |
pSC101 (ts)
|
| Host range: |
Escherichia coli |
| Other genetic elements: |
|
| Strain properties/Comments: |
Derivative of pGB2ts containing the chloramphenicol resistance Ω cassette in place of spec/str cassette. Can not replicate at 42oC but can replicate at 30oC. |
| Author: |
Malgorzata Lobocka |
| Availability: |
restricted |
| Patent: |
|
| Depositor: |
Malgorzata Lobocka |
| Institution/Department: |
|
| Deposition date: |
2006-07-02 23:10+01:00 |
| References: |
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