| Base: |
Plasmid collection |
| Number in base: |
633 |
| Plasmid name: |
pMLO44 |
| Species: |
Escherichia coli K12 |
| Strain: |
DJ125 (=C600 recA) |
| Genotype: |
supE44 hsdR17 thr-1 leuB6 lacY1 thi-1 tonA21 recA
|
| Plasmid Sequence: |
available on request |
| Construction: |
Ligation of EcoRI-EcoRI fragment of HP45 ΩspecR, containing the Ω specR cassette, with the large EcoRI-EcoRI fragment of pMLO7 containing par, ori, rep(ts) and spec/str resistance gene of pGB2ts. Orientation of spectinomycin resistance gene in this plasmid is opposite than in pGB2ts (towards the end of repA(ts) gene.
|
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
cam |
| Antibiotic concetration: |
|
| Plasmid size: |
4.3 kb |
| Origin of replication/Incompatibility group: |
pSC101 (ts)
|
| Host range: |
Escherichia coli |
| Other genetic elements: |
|
| Strain properties/Comments: |
A derivative of pGB2ts in which the specR gene is oppositely oriented than in pGB2ts, and flanked by tandem transcription terminators. It can not replicate at 42oC but can replicate at 30oC. |
| Author: |
Malgorzata Lobocka |
| Availability: |
restricted |
| Patent: |
|
| Depositor: |
Malgorzata Lobocka |
| Institution/Department: |
|
| Deposition date: |
2006-07-02 23:10+01:00 |
| References: |
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