| Base: |
Plasmid collection |
| Number in base: |
634 |
| Plasmid name: |
pMLO46 |
| Species: |
Escherichia coli K12 |
| Strain: |
DJ125 (=C600 recA) |
| Genotype: |
supE44 hsdR17 thr-1 leuB6 lacY1 thi-1 tonA21 recA
|
| Plasmid Sequence: |
available on request |
| Construction: |
Insertion of 1.3 kb BamH1-BamH1 fragment of pRPG18, containing the trpR gene (which encodes the repressor of tryptophan operon) in BamH1 site of pMLO30 (=pACYC184 derivative carrying P1 parA parB genes under the control of promoter-operator region of tryptophan operon [trpPO]).
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| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
cam |
| Antibiotic concetration: |
|
| Plasmid size: |
7.7 kb |
| Origin of replication/Incompatibility group: |
pACYC184
|
| Host range: |
Escherichia coli |
| Other genetic elements: |
|
| Strain properties/Comments: |
A derivative of pACYC184 that carries P1 parA and parB genes under the control of trpPO, and the trpR gene encoding the repressor of trp operon. |
| Author: |
Malgorzata Lobocka |
| Availability: |
restricted |
| Patent: |
|
| Depositor: |
Malgorzata Lobocka |
| Institution/Department: |
|
| Deposition date: |
2006-07-02 23:10+01:00 |
| References: |
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