| Base: |
Plasmid collection |
| Number in base: |
638 |
| Plasmid name: |
pMLO56 |
| Species: |
Escherichia coli K12 |
| Strain: |
DJ125 (=C600 recA) |
| Genotype: |
supE44 hsdR17 thr-1 leuB6 lacY1 thi-1 tonA21 recA
|
| Plasmid Sequence: |
available on request |
| Construction: |
Ligation of large EcoRI-EcoRI fragment of pMLO7 (=derivative of pGB2ts with SphI site replaced by EcoRI site) containing par ori and repA (ts) genes of pGB2ts, with EcoRI-EcoRI fragment of HP45 ΩspecR containing the Ω specR cassette. The orientation of spec/str resistance gene in this plasmid is the same as in pGB2ts, but the gene is flanked by tandem transcription terminators.
|
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
spc, str |
| Antibiotic concetration: |
|
| Plasmid size: |
4.3 kb |
| Origin of replication/Incompatibility group: |
pSC101 (ts)
|
| Host range: |
Escherichia coli |
| Other genetic elements: |
|
| Strain properties/Comments: |
A derivative of pGB2ts containing the spec/str resistance gene (Ω specR cassette) flanked by transcriptional terminators. It can not replicate at 42oC but can replicate at 30oC. |
| Author: |
Malgorzata Lobocka |
| Availability: |
restricted |
| Patent: |
|
| Depositor: |
Malgorzata Lobocka |
| Institution/Department: |
|
| Deposition date: |
2006-07-02 23:10+01:00 |
| References: |
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