| Base: |
Plasmid collection |
| Number in base: |
644 |
| Plasmid name: |
pMLO76 |
| Species: |
Escherichia coli K12 |
| Strain: |
DJ125 (=C600 recA) |
| Genotype: |
supE44 hsdR17 thr-1 leuB6 lacY1 thi-1 tonA21 recA
|
| Plasmid Sequence: |
available on request |
| Construction: |
Replacement of MluI-SalI fragment of pMLO75 containing the 3' end of parB and a fragment of parS (including the IHF binding site and left ParB binding boxes) with similar fragment but mutated by directed PCR mutagenesis. Contains parS' fragment mutated in the IHF binding site and also an additional mutation close to ParB binding site.
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| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
spc, str |
| Antibiotic concetration: |
|
| Plasmid size: |
~6.5 kb |
| Origin of replication/Incompatibility group: |
pSC101 (ts)
|
| Host range: |
Escherichia coli |
| Other genetic elements: |
|
| Strain properties/Comments: |
Derivative of pGB2ts containing the P1 par operon, but with mutant version of parS' (region of parS including IHF bindng site and left ParB-binding boxes). |
| Author: |
Malgorzata Lobocka |
| Availability: |
restricted |
| Patent: |
|
| Depositor: |
Malgorzata Lobocka |
| Institution/Department: |
|
| Deposition date: |
2006-07-02 23:10+01:00 |
| References: |
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