| Base: |
Plasmid collection |
| Number in base: |
646 |
| Plasmid name: |
pB2D |
| Species: |
Escherichia coli K12 |
| Strain: |
DJ125 (=C600 recA) |
| Genotype: |
supE44 hsdR17 thr-1 leuB6 lacY1 thi-1 tonA21 recA
|
| Plasmid Sequence: |
available on request |
| Construction: |
Insertion of 1.47 kb EcoRI-BamH1 fragment of N15cB3Km8 mutant N15 prophage, containing the cB3 allele of cB gene, in place of EcoRI-BamH1 fragment of pUC19 polylinker. In the resultant plasmid, the terminal 71 bp of the insert containing the recognition site for BamH1 are derived not from N15 DNA but from the mini-kan transposon of the mutant.
|
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
amp |
| Antibiotic concetration: |
|
| Plasmid size: |
8.03 kb |
| Origin of replication/Incompatibility group: |
pUC19
|
| Host range: |
Escherichia coli |
| Other genetic elements: |
|
| Strain properties/Comments: |
Derivative of pUC19 containing the cB3 allele of cB gene of N15 prophage. |
| Author: |
Alexander Vostrov |
| Availability: |
restricted |
| Patent: |
|
| Depositor: |
Malgorzata Lobocka |
| Institution/Department: |
|
| Deposition date: |
2006-07-02 23:10+01:00 |
| References: |
Łobocka et al. (1996) J. Bacteriol. 178: 2902-2910. |