| Base: |
Plasmid collection |
| Number in base: |
75 |
| Plasmid name: |
pKG11 |
| Species: |
E. coli |
| Strain: |
DH5α |
| Genotype: |
supE44, lacU169(φ80lacZ ΔM15), hsdR17, recA1, endA1, gyrA96, thi-1, relA1; |
| Plasmid Sequence: |
|
| Construction: |
2.2 kb BamHI insert carrying yeast ERG20 gene tagged on 5` end with ProtA epitope from Staphyloccocus aureus under yeast NOP1promoter in pRS316 plasmid |
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
amp, URA3 |
| Antibiotic concetration: |
|
| Plasmid size: |
7.09 kb |
| Origin of replication/Incompatibility group: |
|
| Host range: |
|
| Other genetic elements: |
|
| Strain properties/Comments: |
plasmid isolated from I-1651 yeast two-hybrid system library |
| Author: |
P. Legrain and M. Fromont, Institute K. Grabinska |
| Availability: |
restricted |
| Patent: |
|
| Depositor: |
K. Grabinska |
| Institution/Department: |
|
| Deposition date: |
1.03.2002 |
| References: |
K. Grabinska, PhD thesis 2002 |