| Base: |
Plasmid collection |
| Number in base: |
770 |
| Plasmid name: |
pADS1 |
| Species: |
Escherichia coli K12 |
| Strain: |
EC17215 |
| Genotype: |
DH5α [recA1 endA1 relA1 thi-1 gyrA-96 hsdR17 ΔlacU169 (φ80 lacZ ΔM15) supE44] |
| Plasmid Sequence: |
. |
| Construction: |
pBEF111 derivative carrying A145T amber mutation in parA gene of P1 par operon under the control of Ptrp promoter; 390 bp HindIII-XhoI fragment of PCR-amplified par region of pBEF111 obtained with primer p488 and mutagenic primer OMLO112 inserted in place of HindIII-XhoI fragment of pBEF111 containing corresponding region of wt par region |
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
amp |
| Antibiotic concetration: |
|
| Plasmid size: |
4.06 kb |
| Origin of replication/Incompatibility group: |
pMB1 (=pBR322) |
| Host range: |
|
| Other genetic elements: |
|
| Strain properties/Comments: |
|
| Author: |
Aneta Dobruk-Serkowska |
| Availability: |
restricted |
| Patent: |
|
| Depositor: |
Aneta Dobruk - Serkowska |
| Institution/Department: |
|
| Deposition date: |
2004-10-20 12:38+01:00 |
| References: |
Aneta Dobruk-Serkowska; Ph.D. thesis Warsaw 2004 |