| Base: |
Plasmid collection |
| Number in base: |
772 |
| Plasmid name: |
pADS22 |
| Species: |
Escherichia coli K12 |
| Strain: |
EC17217 |
| Genotype: |
DJ125 [F- supE44 hsdR17 thi-1 thr-1 leuB6 lacY1 tonA21 recA56] |
| Plasmid Sequence: |
. |
| Construction: |
Ppar mutant of pBEF116; 195 bp HindII-PacI fragment of PCR-amplified Ppar region of pBEF116 obtained with primer OMLO142 and mutagenic primer OMLO143 inserted in place of HindIII-PacI fragment of pBEF116 containing corresponding region of wt Ppar |
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
amp |
| Antibiotic concetration: |
|
| Plasmid size: |
6.2 kb |
| Origin of replication/Incompatibility group: |
pMB1 (= pBR322) |
| Host range: |
|
| Other genetic elements: |
|
| Strain properties/Comments: |
|
| Author: |
Aneta Dobruk-Serkowska |
| Availability: |
restricted |
| Patent: |
|
| Depositor: |
Aneta Dobruk - Serkowska |
| Institution/Department: |
|
| Deposition date: |
2004-10-20 13:25+01:00 |
| References: |
Aneta Dobruk-Serkowska; Ph.D. thesis Warsaw 2004 |