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Central Collection of Strains (IBB)

Base: Plasmid collection
Number in base: 774
Plasmid name: pADS34
Species: Escherichia coli K12
Strain: EC17219
Genotype: DH5Δlac [recA1 endA1 relA1 thi-1 gyrA-96 hsdR17 Δlac supE44]
Plasmid Sequence: .
Construction: pADS33-derivative containing four copies of T1 transcriptional terminator of E. coli rrnB operon (T1x4); MluI-SmaI fragment of pRS552 repaired at MluI end and cloned in PstI-SmaI-cleaved pADS33 repaired at PstI end
Plasmid properties/Comments:
Plasmid map:
Selectable Markers: amp tet
Antibiotic concetration:
Plasmid size: 12.5 kb
Origin of replication/Incompatibility group: pMB1 (=pBR322)
Host range:
Other genetic elements:
Strain properties/Comments:
Author: Aneta Dobruk-Serkowska
Availability: restricted
Patent:
Depositor: Aneta Dobr
Institution/Department:
Deposition date: 2004-10-20 13:37+01:00
References: Aneta Dobruk-Serkowska; Ph.D. thesis Warsaw 2004