| Base: |
Plasmid collection |
| Number in base: |
776 |
| Plasmid name: |
pADS43 |
| Species: |
Escherichia coli K12 |
| Strain: |
EC17221 |
| Genotype: |
DH5Δlac [recA1 endA1 relA1 thi-1 gyrA-96 hsdR17 Δlac supE44] |
| Plasmid Sequence: |
. |
| Construction: |
pRS551 T14-P5parB-lacZ; EcoRI-SphI fragment of pADS10 carrying P5par promoter of parB gene repaired at SphI end and cloned in EcoRI-BamHI-cleaved pRS551 repaired at BamHI end |
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
amp kan |
| Antibiotic concetration: |
|
| Plasmid size: |
12.7 kb |
| Origin of replication/Incompatibility group: |
pMB1 (=pBR322) |
| Host range: |
|
| Other genetic elements: |
|
| Strain properties/Comments: |
|
| Author: |
Aneta Dobruk-Serkowska |
| Availability: |
restricted |
| Patent: |
|
| Depositor: |
Aneta Dobruk - Serkowska |
| Institution/Department: |
|
| Deposition date: |
2004-10-20 13:57+01:00 |
| References: |
Aneta Dobruk-Serkowska; Ph.D. thesis Warsaw 2004 |