| Base: |
Plasmid collection |
| Number in base: |
777 |
| Plasmid name: |
pADS44 |
| Species: |
Escherichia coli K12 |
| Strain: |
EC17222 |
| Genotype: |
DH5Δlac [recA1 endA1 relA1 thi-1 gyrA-96 hsdR17 Δlac supE44] |
| Plasmid Sequence: |
. |
| Construction: |
pRS551 T1x4-P4parB-lacZ; SphI-BseRI fragment of pBEF116 carrying P4par promoter of parB gene obtained by PCR amplification of P4par promoter region with primers: OMLO75 and OMLO76 end repaired and cloned in EcoRI-cleaved and end-repaired pRS551 |
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
amp kan |
| Antibiotic concetration: |
|
| Plasmid size: |
12.7 kb |
| Origin of replication/Incompatibility group: |
pMB1 (=pBR322) |
| Host range: |
|
| Other genetic elements: |
|
| Strain properties/Comments: |
|
| Author: |
Aneta Dobruk-Serkowska |
| Availability: |
restricted |
| Patent: |
|
| Depositor: |
Aneta Dobruk - Serkowska |
| Institution/Department: |
|
| Deposition date: |
2004-10-20 14:10+01:00 |
| References: |
Aneta Dobruk-Serkowska; Ph.D. thesis Warsaw 2004 |