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Central Collection of Strains (IBB)

Base: Plasmid collection
Number in base: 789
Plasmid name: pADS65
Species: Escherichia coli K12
Strain: EC17234
Genotype: DH5Δlac [recA1 endA1 relA1 thi-1 gyrA-96 hsdR17 Δlac supE44]
Plasmid Sequence: .
Construction: pBEF116 derivative carrying par operon of P1 depleted of 158 bp of P1 parA gene from its 5’ end containing four copies of T1 transcription terminator of E. coli rrnB operon (T1x4); 844 bp MluI-SmaI fragment of pRS552 repaired at MluI end and cloned in ClaI-cleaved and end-repaired pADS38
Plasmid properties/Comments:
Plasmid map:
Selectable Markers: amp
Antibiotic concetration:
Plasmid size: 6.9 kb
Origin of replication/Incompatibility group: pMB1
Host range:
Other genetic elements:
Strain properties/Comments:
Author: Aneta Dobruk-Serkowska
Availability: restricted
Patent:
Depositor: Aneta Dobr
Institution/Department:
Deposition date: 2004-10-20 15:22+01:00
References: Aneta Dobruk-Serkowska; Ph.D. thesis Warsaw 2004