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Central Collection of Strains (IBB)

Base: Plasmid collection
Number in base: 802
Plasmid name: pADS83
Species: Escherichia coli K12
Strain: EC17247
Genotype: DH5Δlac [recA1 endA1 relA1 thi-1 gyrA-96 hsdR17 Δlac supE44]
Plasmid Sequence: .
Construction: pRS552 T1x4-Ppar-parA’+18-‘lacZ; EcoRI-BamHI fragment of PCR-amplified Ppar-parA+18 region of pBEF116 obtained with primers: pOMLO142 and pOMLO184 cloned in EcoRI-BamHI cleaved pRS552
Plasmid properties/Comments:
Plasmid map:
Selectable Markers: amp kan
Antibiotic concetration:
Plasmid size: 12.6 kb
Origin of replication/Incompatibility group: pMB1
Host range:
Other genetic elements:
Strain properties/Comments:
Author: Aneta Dobruk-Serkowska
Availability: restricted
Patent:
Depositor: Aneta Dobr
Institution/Department:
Deposition date: 2004-10-21 10:34+01:00
References: Aneta Dobruk-Serkowska; Ph.D. thesis Warsaw 2004