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Central Collection of Strains (IBB)

Base: Plasmid collection
Number in base: 86
Plasmid name: pYEF1HCVhel
Species: Escherichia coli
Strain: DH5α
Genotype: F-, f80dlacZDM15 Dlac(lac ZYA-argF)U169, deoR, hsdR17 (rk-,mk-), recA1, endA1, gyrA46, pho A, sup E44, thi-1, relA1
Plasmid Sequence:
Construction: derivative of the pYEF1 plasmid; 1500 bp insert, coding for the RNA helicase of the HCV virus, was cloned by the homologous recombination with the vector digested with NotI and EcoRI
Plasmid properties/Comments:
Plasmid map:
Selectable Markers: amp,URA3
Antibiotic concetration:
Plasmid size: 8509 bp
Origin of replication/Incompatibility group: 2 &mikro;m
Host range: E.coli, S.cerevisiae
Other genetic elements:
Strain properties/Comments:
Author: Boguszewska-Chachulska A.M.
Availability: restricted
Patent:
Depositor: Iwona Rosa
Institution/Department:
Deposition date: 29.03.2002
References: