| Base: |
Plasmid collection |
| Number in base: |
90 |
| Plasmid name: |
YCpMM3 (YRp14/CEN4/H4-ARS) |
| Species: |
Escherichia coli |
| Strain: |
DH5α |
| Genotype: |
F-, f80dlacZDM15 Dlac(lac ZYA-argF)U169, deoR, hsdR17 (rk-,mk-), recA1, endA1, gyrA46, pho A, sup E44, thi-1, relA1 |
| Plasmid Sequence: |
|
| Construction: |
derivative of pYRP14 |
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
amp, URA3 |
| Antibiotic concetration: |
|
| Plasmid size: |
8000 bp |
| Origin of replication/Incompatibility group: |
Col E1 rep ori and autonomic replication sequence (ARS) bound to the H4 histone (H4-ARS) |
| Host range: |
E.coli, S. cerevisiae |
| Other genetic elements: |
centromere from the chromosome IV (CEN4) and suppressor tRNA (SUP11) gene - suppression of the ochre type nonsense mutation |
| Strain properties/Comments: |
|
| Author: |
Hieter, P., C. Mann, M. Snyder and R. Davis |
| Availability: |
restricted |
| Patent: |
|
| Depositor: |
Iwona Rosa |
| Institution/Department: |
|
| Deposition date: |
29.03.2002 |
| References: |
Hieter, P., C. Mann, M. Snyder and R. Davis, (1985), Cell 40: 381–392. |